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Knockdown of coatomer protein complex subunit beta 2 (COPB2) promotes the exit of Yes-associated protein 1 (YAP1) from the nucleus and affects its stability. (A) Representative images of immunofluorescent staining for YAP1 distribution. (B) Representative pictures of Western blotting analysis of pLATS1 and <t>pYAP1</t> in Huh7 and SK-hep1 cells transfected with si-NC and si-COPB2. All data are displayed as mean ± standard deviation (SD). ** p < 0.01.
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Knockdown of coatomer protein complex subunit beta 2 (COPB2) promotes the exit of Yes-associated protein 1 (YAP1) from the nucleus and affects its stability. (A) Representative images of immunofluorescent staining for YAP1 distribution. (B) Representative pictures of Western blotting analysis of pLATS1 and pYAP1 in Huh7 and SK-hep1 cells transfected with si-NC and si-COPB2. All data are displayed as mean ± standard deviation (SD). ** p < 0.01.

Journal: Oncology Research

Article Title: COPB2 promotes hepatocellular carcinoma progression through regulation of YAP1 nuclear translocation

doi: 10.32604/or.2025.058085

Figure Lengend Snippet: Knockdown of coatomer protein complex subunit beta 2 (COPB2) promotes the exit of Yes-associated protein 1 (YAP1) from the nucleus and affects its stability. (A) Representative images of immunofluorescent staining for YAP1 distribution. (B) Representative pictures of Western blotting analysis of pLATS1 and pYAP1 in Huh7 and SK-hep1 cells transfected with si-NC and si-COPB2. All data are displayed as mean ± standard deviation (SD). ** p < 0.01.

Article Snippet: The following antibodies were used in this study: COPB2 (ab229639, 1:1000, rabbit, Abcam, Cambridge, UK), YAP1 (ab205270, 1:500, rabbit, Abcam, Cambridge, UK), pLATS1 (8654T, 1:500, rabbit, Cell Signaling Technology, Danvers, MA, USA), pYAP1 (49115, 1:500, rabbit, Cell Signaling Technology, Massachusetts, USA), and GAPDH (ab8245, 1:5000, mouse, Abcam, Cambridge, UK).

Techniques: Knockdown, Staining, Western Blot, Transfection, Standard Deviation